H1840-45C
CagA (H. pylori Cytotoxin Associated Gene A Protein)
Size
£646.00
- SKU:
- H1840-45C
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Highly immunogenic 32Kd CagA fragment, recombinant
- Clone:
- 10g180
- Format:
- Supplied as a lyophilized powder from 0.01M PBS, pH 7.0. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Helicobacter pylori (H. pylori), a spiral rod shaped gram negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H.pylori isolatesfrom carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H.pylori strains. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: Detects a band at 32kD Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Matched Pair: Capture: H1840-45C Dectection: H1840-45E, H1840-45F or H1840-45G Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice