Heat Shock Protein 20 (HSP20, Hspb6, Heat Shock protein, alpha-crystallin-related, B6, Heat shock 20kD-like protein p20, Heat shock protein beta-6, HspB6, p20)

Size

£563.00

SKU:: H1834
Application:: WB
Purity:: Pruified by peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Rat skeletal muscle Hsp20
Format:: Supplied as a liquid in PBS, pH 7.0, 3% BSA, 0.09% sodium azide.
Extra Details:: Hsp20 is a member of the family of small heat shock proteins (sHsps), with homology to Hsp25, Hsp27, and alphaB-crystallin. Highest expression of Hsp20 is found in skeletal, smooth, and cardiac muscle. Under heat shock, Hsp20 translocates from the cytosol to the nucleus, although the amount of Hsp20 in the cell does not increase like other Hsps. Hsp20 oligomerization and interaction with polymeric actin filaments is regulated via phosphorylation by PKA and PKG Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot (Colormetric): 1:1000 detects a band at ~20kD Optimal dilutions to be determined by the researcher. Positive Controls: HeLa Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.