H1834-55J

Heat Shock Protein 90, beta (HSP90b)

Size

£837.00

SKU:: H1834-55J
Purity:: Supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HSP90 beta.
Clone:: 6A93
Format:: Supplied as a liquid, 0.09% sodium azide.
Extra Details:: The 90kD heat shock protein (HSP90) is a molecular chaperone that keeps a target protein in a folding-competent state. Hsp90 is ubiquitously expressed and abundant, and it participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase. In its ATP-bound state, Hsp90 interacts with the co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. Hsp90 is highly conserved between species; mammalian proteins demonstrate 60% amino acid identity with the corresponding yeast and 78% identity with the corresponding Drosophila proteins. Two isoforms of HSP90, alpha and beta, are expressed in the cytosolic compartment.6 The amino acid sequence of human HSP90 alpha is 85% homologous to HSP90 beta, but HSP90 alpha exists predominantly as a homodimer while HSP90 beta exists mainly as a monomer. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:100-1:1000 Immunoprecipitation: 100ul/test Western Blot: 1:100 Optimal dilutions to be determined by the researcher. Positive Controls: HeLa, T47D, A549, and PC-3 cell lysates and rabbit reticulocyte lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice