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H1834-55E

Heat Shock Protein 90 (HSP90)

Size

£940.00

SKU:
H1834-55E
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Purified human Hsp90.
Format:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Extra Details:
The 90kD molecular chaperone family comprises several proteins including the 90kD heat shock protein, Hsp90 and the 94kD glucose-regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90alpha and Hsp90beta which are encoded by separate genes. The amino acid sequence of human and yeast Hsp90alpha is 85% and 90% homologous to that of Hsp90 beta respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions which have been shown to contain independent chaperone sites with different substrate specificity. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 (ECL). Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Positive Controls: Native human Hsp90 Protein, recombinant human Hsp90a, HeLa Cell Lysate, 3T3, PC-12, RK-13 Recommended Tissue for Immunohistochemistry: Human spleen Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice