H1834-55C
Heat Shock Protein 90 (HSP90)
Size
£607.00
- SKU:
- H1834-55C
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Native human Hsp90 protein.
- Clone:
- 4E130 (16F1)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- The 90kD molecular chaperone family comprises several proteins including the 90kD heat shock protein, Hsp90 and the 94kD glucose-regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90alpha and hsp90beta which are encoded by separate genes. The amino acid sequence of human and yeast Hsp90alpha is 85% and 90% homologous to that of Hsp90beta respectively (1). All known members of the Hsp90 protein family are highly conserved. These proteins account for 1-2% of all cellular proteins in most cells. Hsp90 fights the deleterious consequences of protein unfolding, however, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signalling and cell cycle control. In this context several key regulatory proteins have been identified as substrates of Hsp90 (4). It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways. Recent studies indicate that when Drosophilia Hsp90 buffering is compromised by temperature etc., cryptic variants are expressed and selection can lead to the continued expression of these traits, even if Hsp90 function is restored (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot (Colorimetric): 1:1000 Optimal dilutions to be determined by researcher. Positive Controls: H1831-57: Heat Shock Protein 90, Human (HSP90) H1832-85:Heat Shock Protein 90 alpha, Recombinant, Human HeLa Heat Shocked Cell Lysate (HSP90a) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice