H1834-30

Heat Shock Protein 65, Mycobacterial (HSP65)

Size

£570.00

SKU:: H1834-30
Application:: WB
Purity:: Purified by ammonium sulfate precipitation from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to M. bovis Hsp65.
Clone:: 4E123
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Hsp65, a 65kD protein isolated from Mycobacterium bovis BCG, is a member of the hsp60 family of heat shock proteins. Sequence comparison of Hsp65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively. Mycobacterium bovis BCG Hsp65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot (Colorimetric): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.