Heat Shock Protein 27 (HSP27)

Size

£704.00

SKU:: H1834-05B
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human HSP27, fused to His-Tag
Format:: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Extra Details:: HSP27, a member of the small heat shock protein (sHSP) family, is an abundant and ubiquitously expressed molecular chaperone found in both normal and cancerous human cells. Structurally, HSP27 features a conserved α-crystallin domain at the C-terminus and a less conserved N-terminal WD/EPF domain, which is essential for forming high-molecular-weight oligomers. These oligomers, composed of 8–40 monomers, are critical for HSP27’s chaperone activity—larger assemblies exhibit strong anti-aggregation properties, while dimers are inactive. Under non-stress conditions, HSP27 resides in the cytoplasm but rapidly translocates to the nucleus in response to cellular stress, where it may help stabilize DNA and nuclear membranes. Functioning as an ATP-independent chaperone, HSP27 prevents protein aggregation and stabilizes partially unfolded proteins, facilitating their refolding via the HSP70 complex. In neuroscience, HSP27 is increasingly recognized for its role in protecting neurons from proteotoxic stress, oxidative damage, and apoptosis—key features of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. It inhibits apoptotic signaling by blocking the cytochrome c/Apaf-1/procaspase-9 complex and may also influence cytoskeletal dynamics through interactions with actin and myosin. HSP27 expression is tightly regulated by phosphorylation, which promotes the formation of large, active oligomers. Its involvement in stress response, apoptosis inhibition, and cell differentiation highlights its therapeutic potential in neurodegenerative disease research and intervention. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000 Immunocytochemistry/Immunofluorescence: 1:250 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice