H1833
HE Pervanadate Control Lysate
Size
£358.00
- SKU:
- H1833
- Application:
- WB
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).
- Extra Details:
- Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. Human aortic endothelial cells treated with pervanadate for 30 minutes display a significant increase in tyrosine phosphorylation as shown by western blotting using anti-Phosphotyrosine. Confluent cultures of human aortic endothelial cells were serum starved overnight. Cells were then either left untreated or treated with pervanadate (1mM) for 30 minutes at 37°C. Cells were lysed in 1% SDS, 1mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. Applications: SDS-PAGE/Western blotting 20ml/well. End user should determine optimal quantity for their particular applications and experiments.
- Shipping Conditions:
- Blue Ice