H1833-02
Heat Shock Protein 105 (HSP105, HSPH1)
Size
£837.00
- SKU:
- H1833-02
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant rat HSPH1 (rrHSPH1; aa595-733)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. Alpha and beta have calculated molecular weights of 96.4 kD and 92 kD, respectively, but an apparent molecular weight of 105-110 kD in SDS-PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively. Applications: Western Blot: An antibody concentration of 0.1ug/ml is recommended. Optimal dilutions should be determined by the individual laboratory. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice