H1831-56
Heat Shock Protein 90 (HSP 90)
Size
£940.00
- SKU:
- H1831-56
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full-length protein purified from chicken brain. Mr 90kD.
- Clone:
- D7a
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 4ug immunoprecipitates HSP90 from 500ug of 3T3 RIPA lysate. Western Blot: 0.5-2ug/ml detects HSP90 in RIPA lysates from mouse 3T3 fibroblast and human A431 cells. Western Blot Analysis 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-mouse HSP 90 (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: 3T3 cell lysate. Add 2.5ul of 2-mercaptoethanol/ 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice