H1831-12
Heat Shock Protein 70 (HSP70, HSP72) (Biotin)
Size
£691.00
- SKU:
- H1831-12
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human HSP70
- Clone:
- 4E108 (C92F3A-5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
- Extra Details:
- The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78kD, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. Applications: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA, Immunocytochemisty/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot (Colorimetric): 1:1000 Optimal dilutions to be determined by researcher. Positive Controls: Recombinant Human Hsp70 Protein: H1830-92 HeLa Heat Shocked Cell Lysate: H1838-01 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice