H1830-95X
Heat Shock Protein 70 (HSP70) (BSA & Azide Free)
Size
£1,023.00
- SKU:
- H1830-95X
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HSP70 protein from HeLa cells.
- Clone:
- 0.N.324
- Format:
- Supplied as a liquid in PBS, pH 7.4.
- Extra Details:
- Heat shock proteins, HSP’s or stress response proteins SRP’s, are synthesized in a variety of environmental and patholphysiological stressful conditions. Many HSP’s are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native & denatured): 2ug antibody/mg protein lysate using Protein A. Western Blot: 0.5-1ug/ml for 2hrs at RT. Immunohistochemistry (Frozen & formalin/paraffin): 2-4ug/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: BT474 or HeLa cells, breast carcinoma. Cellular Localization: Nuclear and cyotplasmic Molecular Weight of Antigen: Inducible: 72kD Constitutive: 73Kd Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice