H1830-92S

Heat Shock Protein 70, Geodia cydonium (HSP70, GCHSP70)

Size

£351.00

SKU:: H1830-92S
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: HSP70 from Geodia cydonium
Clone:: IVF5
Format:: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Extra Details:: Applications: Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blotting: 1:500-1:1000. Extracts from cadmium chloride-exposed Suberites domuncula were separated by size and transferred to membranes. The blots were incubated with antibody IVF5. Immunohistochemistry: 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.