H1830-70J

Heat Shock Protein 60 (HSP60, GroEL, Chaperonin 60, cpn60)

Size

£416.00

SKU:: H1830-70J
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa1-573 from human HSP60, expressed in E. coli.
Clone:: 2E4
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide 10% glycerol.
Extra Details:: Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kD mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding. Applications: Suitable for use in ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:3000 Western Blot: 1:1000-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.