H1830-52
Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)
Size
£863.00
- SKU:
- H1830-52
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH.
- Clone:
- 2T7 (JBW502)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
- Extra Details:
- Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-0.5ug/ml detects phosphorylated HSP27 in 20ug of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes. Untreated and heat shock, and arsenite treated MCF-7 cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with 0.05ug/ml of H1830-52. Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice