H1830-48-APC

Heat Shock Protein 20 (HSP20) (APC)

Size

£1,113.00

SKU:: H1830-48-APC
Purity:: Purified by Protein A affinity chromatography from ascites. (≥90%)
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HSP20.
Clone:: 2B38
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and alphaB-crystallin and may form different hetercomplexes with these proteins. The specific physiologic function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. Hsp20 can be phosphorylated in vitro by cAMP-dependant protein kinase (PKA) and cGMP-dependant protein kinase (PKG). Phosphorylated Hsp20 is proposed to interact with monomeric actin and alpha-actinin whereas dephosphorylated Hsp20 binds polymeric actin filaments. Phosphorylated Hsp20 is also associated with vasorelaxation. Dissociated Hsp20, but not aggregated Hsp20, suppresses platelet aggregation, and human platelets show specific binding sites for Hsp20. Elevated levels of dissociated Hsp20 have also been found in the plasma of cardiomyopathic hamsters. Hsp20, which immediately responds to pathological events, may act extracellularly as a regulator of platelet functions. Applications: Suitable for use in FLISA, Western Blot and Immunofluroescence. Other applications have not been tested. Recommended Dilutions: Immunofluorescence: Cell culture staining Optimal dilutions to be determined by the researcher. Hybridoma: Sp 2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Allophycocyanin (APC) is light sensitive. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice