H1827-55P

HDAC5, Recombinant, Human (HD5, FLJ90614, KIAA0600, NY-CO-9, Histone Deacetylase 5)

Size

£1,022.00

SKU:: H1827-55P
Molecular Weight:: 51
Purity:: ≥ 50% by SDS-PAGE
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM sodium chloride, 0.05% Tween 20, 10% glycerol.
Extra Details:: Human HDAC5 (GenBank Accession No. NM_001015053), amino acids 657-1123 with C-terminal His tag, expressed in baculovirus expression system. Specific Activity: As Reported Assay Condition: 25mM Tris/Cl, pH 8.0, 137mM sodium chloride, 2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA, 30uM HDAC substrate and 0.4ng/ul HDAC5 for 30 minutes at 30ºC. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.