H1827-55
HDAC3 (Histone Deacetylase 3)
Size
£927.00
- SKU:
- H1827-55
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated, 18 residue synthetic peptide corresponding to amino acids 411–428 of human HDAC3 (NEFYDGDHDNDKESDVEI). The immunizing sequence is identical for chicken and for the first 14 residues in mouse.
- Format:
- Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide containing 30% glycerol.
- Extra Details:
- Acetylation and deacetylation of core histone lysine residues, by histone acetyltransferases (HAT) and histone deacetylases (HDAC) respectively, have been shown to be necessary for some crucial nuclear events such as gene transcription, DNA replication and repair. HDACs are found in multi-protein complexes with transcriptional co-repressors such as mSin3, RbAp46/48, SMRT, and NCoR. HDAC3, which is over 50% identical to both HDAC1 and HDAC2, is ubiquitously expressed and exhibits histone deacetylase activity, represses transcription and associates with the transcription factor YY1. The identification of a family of functionally redundant HDACs suggests each may have a unique function, supported by the sequence divergence of the carboxy-termini. Applications: Immunoblot Analysis: 1–4ug/ml detected HDAC3 from a HeLa RIPA cell lysate (1 hour exposure). 4ug/ml detected HDAC from mouse WEHI and rat L6 RIPA cell lysates (30 minute exposure). Immunoblot Analysis HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC3 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice