H1827-53B

HDAC2, Recombinant, Human (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)

Size

£1,046.00

SKU:: H1827-53B
Molecular Weight:: 60
Purity:: ~ 70% by SDS-PAGE
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris, pH 8.0, 100mM sodium chloride, 2.2mM KCl, 0.04% Tween 20, 20% glycerol.
Extra Details:: Human, recombinant, full length with C-terminal His tag expressed in Sf9 cells. Applications: Suitable for use in enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Specific Activity: ~400pmol/min/ug Recommended Dilution: Assay Conditions: 50mM Tris-HCl, pH 8.0, 137mM sodium chloride, 2.7mM of KCl, 1mM MgCl2, 20uM substrate, 5ug BSA, H1827-53B. Incubate at 37ºC for 30 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.