H1827-50E

HDAC1, Recombinant, Human, His-FLAG (Histone Deacetylase 1)

Size

£1,046.00

SKU:: H1827-50E
Molecular Weight:: 56
Purity:: ≥50% by SDS-PAGE
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 80ng/ul FLAG peptide, 20% glycerol
Extra Details:: Description: Human HDAC1 (GenBank Accession No. NM_004964), full length with C-terminal His-FLAG tag. Specific Activity: ~400pmol/min/ug Assay Conditions: 25mM Tris/Cl, pH 8.0, 137mM sodium chloride,2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA, 20uM BPS HDAC substrate, HDAC1 Incubation conditions: 30 minutes at 37ºC followed by treatment with 50ul of HDAC developer, Catalog #H5109-66B for 15 minutes at RT Applications: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.