H1827-28
HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)
Size
£1,244.00
- SKU:
- H1827-28
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- GST fusion protein corresponding to aa119-155, HGHLH DSAEE RRLIA EGDAS PGEDR TPPGL AAEPE RP of human HCN4.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
- Extra Details:
- Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry: mouse brain sections Note: Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal dilutions to be determined by the researcher. Control Antigen Included: H1827-28A: HCN4, Control Antigen Reconstitute with 100ul sterile PBS. For Positive Control in Western Blot, use 20ng of protein per Minigel lane. For negative control preincubate 3ug of fusion protein with 1ug antibody for 1hour at RT. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice