H0010-20
H Type 2 Antigen
Size
£843.00
- SKU:
- H0010-20
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Mucin isolated from a mucinous colonic adenocarcinoma
- Clone:
- 19-OLE
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- H type 2 antigen is the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Ethanol fixed, frozen sections and ethanol or formalin fixed paraffin sections. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min Immunocytochemistry: Strongly positive on ethanol fixed epithelial cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice