G9028

Guanidinoacetate N-methyltransferase isoform, Recombinant Human, aa1-256, His-Tag (GAMT)

Size

£528.00

SKU:: G9028
Molecular Weight:: 28.4
Purity:: ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 10% glycerol.
Extra Details:: Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant corresponding to human GAMT, his-tagged, expressed in E. coli. AA sequence: MGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Shipping Conditions:: Dry Ice