G9005-75

GS28 (GOS28, p28)

Size

£601.00

SKU:: G9005-75
Application:: WB
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat liver Golgi-enriched membranes.
Clone:: 4E99
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol.
Extra Details:: GS28, also known as p28 or GOS28, is a 28kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport. The N-terminal of GS28/GOS28 is exposed to the cytosol and is anchored to the cis Golgi via a 20 amino acid carboxyl-terminal hydrophobic tail. GS28 co-immunoprecipitates complexes consisting of syntaxin 5, rbet1, membrin, rsec22, and rsly1, and is therefore implicated in ER-to-Golgi or intra-Golgi vesicle transport. ATP hydrolysis by NSF in the presence of alpha-SNAP causes the dissociation of GOS28 and syntaxin 5 complex. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot (ECL): 1:1000 Optimal dilutions to be determined by the researcher. Positive Controls: Rat Brain Tissue Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.