G9000-22A

Growth Hormone, Human (hGH)

Size

£512.00

SKU:: G9000-22A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant hGH.
Clone:: 10K232
Format:: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
Extra Details:: Growth Hormone (GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191aa residues with 2 disulfide bridges. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites. Application: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20 PBS to1mg/ml Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.