G8990-05
Growth Associated Protein (GAP)-43/b-50
Size
£991.00
- SKU:
- G8990-05
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GAP-43 purified from rat brain. Species sequence homology: human
- Clone:
- 2Q55
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
- Extra Details:
- GAP-43 is a protein expressed at elevated levels by developing or regenerating neurons during axon growth. While this neuron-specific membrane protein is a component of axonal growth cones, it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 0.1-0.5ug/ml on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X- 100 to incubation buffer will increase cellular permeability. Western Blot: will detect a single 43-48kD band in western blots of membrane fractions of growing neurons. Immunoprecipitation: 5-10ug/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice