G8990-01J

GAP43 (Neuromodulin, Axonal Membrane Protein GAP-43, Growth-associated Protein 43, Neural Phosphoprotein B-50, pp46)

Size

£512.00

SKU:: G8990-01J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: GAP-43 from neonatal rat forebrain membranes.
Clone:: 10K231
Format:: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute in sterile ddH2O, PBS.
Extra Details:: GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells.It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (paraffin, frozen): 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 200ul sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.