G8990-01B
Growth Associated Protein 43, phosphorylated (Ser41) (GAP43, Neuromodulin, PP46, p57, B-50, F1, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50)
Size
£761.00
- SKU:
- G8990-01B
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to KLH. Species Sequence Homology: bovine, canine, chicken, human, monkey, Xenopus and zebrafish
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser-41 by protein kinase C modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002). Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Detects a band of ~50kD in rat cortical lysates. Dot Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice