G8989-51

GDF15 (GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal inflammatory drug)-activated protein 1)

Size

£642.00

SKU:: G8989-51
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-QKTDTGVSLQTYDD from the C-terminus of human GDF15 (NP_004855.2) Species Sequence Homology: mouse, rat
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein. Applications: Suitable for use in Peptide ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA: 1:2000 Western Blot: 0.1-0.3ug/ml detects ~35kD band observed in human prostate lysates (calculated MW of 34.1kD according to NP_004855.1). In transfected HEK293 transiently expressing GDF15 a band of ~35kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice