G8972

GRK 2,3 (betaARK 1,2)

Size

£825.00

SKU:: G8972
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partial GST fusion protein corresponding to the GRK3 (bARK2) carboxyl terminus (aa 467-688). This immunogen shares homology with GRK2 (bARK1). The minimal epitope recognized by the antibody is aa 483-485.
Clone:: 0.T.54 (C5/1.1)
Format:: Supplied as a liquid in in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: This antibody can be used to immunoprecipitate GRK2/3 from transfected cells. Western Blot: 0.1-1ug/ml. Detects GRK2/3 in RIPA lysates from mouse 3T3 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8972 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilution determined by the researcher. Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Positive Control Included: F4209: Fibroblast Cell, 3T3/A31. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice