G8967-80
Griffonia simplicifolia GS II
Size
£390.00
- SKU:
- G8967-80
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 0.5mM calcium chloride. Reconstitute with buffer of choice. Note: Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitiate.
- Extra Details:
- The seeds of Griffonia simlicifolia conain four distinct lectins, 30,000D each. Two of these lectins, referred to as GS-I and GS-II have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer but it is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only a terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding. Source: Bandeiraea Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200mM N-acetylglucosamine Activity: 5-10ug/ml will agglutinate Tk polyagglutinaable cells. Inhibitory Carbohydrate: N-acetylglucosamine Carbohydrate Specificity: Terminal a- or b- N- Acetylglucosamine. The specific linkage of N-Acetylglucosamine to the subterminal carbohydrate plays an important role in lectin binding. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice