Green Fluorescent Protein (Aequorea victoria) (GFP)

Size

£800.00

SKU:: G8965-13A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Full length aa(246) derived from the jellyfish Aequorea victoria.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Extra Details:: GFP is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Chicken IgY lacks the classic "Fc" domain and does not bind to mammalian IgG Fc receptors. Thus resulting in lower backgrounds for western blotting, ELISA and Immunohistochemistry. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000-1:120,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.