G8965-12E
Green Fluorescent Protein (GFP)
Size
£537.00
- SKU:
- G8965-12E
- Application:
- WB
- Purity:
- Purified by Protein G affinity Chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa101-117 (KDDGNYKTRAEVKFEGD) of GFP. This sequence is 100% homologous to YFP and CFP.
- Format:
- Supplied as a liquid in PBS, 0.2% BSA, 0.05% sodium azide.
- Extra Details:
- Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and the demonstration that GFP can be expressed as a functional transgene, GFP has become a unique new tool with exciting applications in cell, developmental and molecular biology. GFP is an ideal fluorescent probe: its fluorescence is not species specific (it has been expressed from bacteria, yeast, plant to mammalian cells); it can fuse with proteins of interest without interfering significantly with their assembly or function. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Transfected cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice