G8965-10E
Green Fluorescent Protein, Recombinant (GFP)
Size
£313.00
- SKU:
- G8965-10E
- Application:
- WB
- Molecular Weight:
- 28
- Purity:
- Purified using Ni-NTA agarose (~70%).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in PBS, 20% glycerol.
- Extra Details:
- GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Full -length recombinant protein corresponding to Green Fluorescent Protein with N-terminal His-tag, expressed in E. coli. Accession: AAA27722. Applications: Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of fluorometers and microinjection into cells and tissues. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice