Green Fluorescent Protein (GFP)

Size

£991.00

SKU:: G8965-06
Purity:: Purified by ammonium sulfate precipitation and PEG.
Storage Conditions:: 4°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: His-tagged green fluorescent protein of Aequorea victoria.
Format:: Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects Green Fluorescent Protein from 50ug of rat brain lysate containing 25ng of purified Green Fluorescent Protein. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Positive Control: rat brain lysate, cells expressing Green Fluorescent Protein Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice