G8965-01

Green Fluorescent Protein (GFP)

Size

£356.00

SKU:: G8965-01
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant His-GFP full length fusion protein (Aequorea victoria).
Clone:: GF200
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 ELISA: 1:2000-1:20,000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse GF200 Source: Ascites Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.