G8960-02A

Grb2 (GRB2, Growth Factor Receptor-bound Protein 2, ASH, ASH Protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, Abundant SRC Homology, Epidermal Growth Factor Receptor-binding Protein GRB2, Growth Factor Receptor-bound Protein 3)

Size

£642.00

SKU:: G8960-02A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: C-PRNYVTPVNRNV, C Terminus
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000. Western blot: Approx 30kD band seen in Jurkat lysate. Recommended for use at 1-3ug/ml. Staining (2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Immunohistochemistry: 0.1-0.3ug/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.