G8957-15E
Granzyme B (C11, Cathespin G Like 1, CCPI,CGL 1, CGL1, CSP B, CSPB, CTLA 1, CTLA1, CTSGL 1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte protease, Protease Serine B, SECT) (Biotin)
Size
£728.00
- SKU:
- G8957-15E
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Granzyme B
- Clone:
- 3H2181 (GB10)
- Format:
- Supplied as a liquid in TBS, 1% BSA, 0.001% Thimerosal. Labeled with Biotin.
- Extra Details:
- Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis. Granzyme B is expressed within the granules of cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA: 1ug/ml Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Matched Pair: Capture: G8957-15B Detection: G8957-15E Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice