G8957-15B-ML550
Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte protease, Protease Serine B, SECT) (MaxLight 550)
Size
£819.00
- SKU:
- G8957-15B-ML550
- Purity:
- Purified by Protein A affinity chromatography from tissue cuture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human Granzyme B.
- Clone:
- 3H2182 (GB11)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis. Granzyme B is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and FLISA. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Membrane permeabilization is required. Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice