G8957-01H

Granzyme B (GRB, GZMB, C11, Cathespin G-like 1, CCPI, CGL1, CGL-1, CSPB, CSP-B, CTLA1, CTLA-1, Cytotoxic T Lymphocyte-associated Serine Esterase 1, CTSGL1, Cytotoxic T Lymphocyte Proteinase 2, Fragmentin 2, Fragmentin-2, Granzyme 2, Human Lymphocyte Protein, HLP, Lymphocyte Protease, Protease Serine B, SECT, T-cell Serine Protease 1-3E)

Size

£793.00

SKU:: G8957-01H
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: GrB-7
Format:: Supplied as a liquid, 0.1% BSA, 0.02% sodium azide
Extra Details:: Granzyme B is involved in target cell apoptosis during lymphocyte mediated cyto-toxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell will lead to induction of DNA fragmentation and apoptosis of the target cell. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:20 Stains granzyme B expressing lymphocytes in sections of sublimate formaldehyde as well as formalin fixed paraffin embedded tissues. Does not react with other cell types. Cannot be used on frozen sections. Pretreatment with 0.1M sodium citrate, for 10 min at 100°C in a microwave oven is advised. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.