G8957-01C
Granzyme B (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, HLP, Human lymphocyte protein, Lymphocyte protease, Protease Serine B, SECT)
Size
£876.00
- SKU:
- G8957-01C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly19-Tyr247 from human Granzyme B, expressed in NS0 cells.
- Clone:
- 8H156
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Granzyme B, also known as CTLA-1, is a serine protease found in the cytotoxic granules of cytotoxic T lymphocytes and NK cells and in articular chondrocytes. Granzyme B enters target cells through perforin channels and induces caspase-dependent apoptosis. It is upregulated in rheumatoid arthritis and cleaves vitronectin, fibronectin, and laminin. Human Granzyme B is synthesized as a precursor with a signal peptide (aa 1-18), a two amino acid propeptide, and a mature chain (aa 21-247). Mature human Granzyme B shares 69% amino acid sequence identity with mouse Granzyme B. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml NK-92 human natural killer lymphoma cell line. Flow Cytometry: 2.5ug/10^6 NK-92 human natural killer lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/mL Immersion fixed human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice