G8956-02A
Granzyme A
Size
£863.00
- SKU:
- G8956-02A
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Cys26-Val262 from human Granzyme A, expressed in NS0 cells.
- Clone:
- 7H128
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Granzyme A, also known as CTLA-3, is the most abundant serine protease found specifically in the cytotoxic granules of cytotoxic T lymphocytes and NK cells. It is released during cellular activation and enters target cells through perforin channels. Granzyme A mediates target cell killing inducing caspase-independent cell death. Human granzyme A is synthesized as a precursor with a signal peptide, a two amino acid propeptide, and a mature chain. Mature human granzyme A shares 71% amino acid sequence identity with mouse granzyme A. Applications: Suitable for use in Immunoprecipitation, Immunocytochemistry, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Granzyme A. Immunocytochemistry: 8-25ug/ml using immersion fixed human peripheral mononuclear cells (PBMCs) Flow Cytometry (Intracellular Staining): 2.5ug/10e6 cells using NK-92 human natural killer lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice