G8950-51A

G-CSF Receptor, Recombinant, Human (G-CSFR, GCSFR, CSF3R, Granulocyte Colony-stimulating Factor Receptor, G-CSF-R, CD114)

Size

£832.00

SKU:: G8950-51A
Molecular Weight:: 95105
Purity:: ≥97%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1.0 EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 50ug BSA. Reconstitute with 500ul sterile PBS, >0.1% human or bovine serum albumin.
Extra Details:: Granulocyte Colony Stimulating Factor (GCSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. GCSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human GCSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by GCSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human GCSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of GCSF R that differ in their Cterminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble GCSF R in human serum has not been reported (1). Mutations have been found in the gene encoding GCSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse GCSF R have a homology of 62.5%. GCSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of GCSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), srcrelated protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon GCSF stimulation (1). Recombinant protein corresponding to Glu25-Pro621 from human G-SCFR from murine myeloma cell line, NS0-derived. Swiss/UniProt Accession: Q99062. SDS-PAGE: ~95-105kD, reducing conditions Activity: Measured by its ability to inhibit the G-CSF-induced proliferation of NFS-60 mouse myeloid cells. The ED50 is typically 1-4ug/ml in the presence of 0.125ng/ml of rhG-CSF. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice