G8950-50L
G-CSF Receptor (G-CSFR, GCSFR, CSF3R, Granulocyte Colony-stimulating Factor Receptor, G-CSF-R, CD114)
Size
£927.00
- SKU:
- G8950-50L
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to human GCSFR, expressed in E.coli.
- Clone:
- 11C109
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- GCSF (G-CSF, CSF3), is a glycoprotein that is produced and secreted by macrophages stimulated with endotoxin. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863aa (23aa signal peptide, 604aa extracellular domain, a 26aa transmembrane domain, and a 183aa cytoplasmic domain). As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C- terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported. Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation. Applications: Suitable for use in Western Blot, ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml (chemiluminescence) ELISA: 1:10,000-1:100,000 (using 50-100ng of control protein/well). Flow Cytometry: ~20-30ug/ml in human PBMC cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice