G8950-10A

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pluripoietin Alpha, Sargramostim)

Size

£544.00

SKU:: G8950-10A
Molecular Weight:: 14.48
Purity:: ≥98% by HPLC and SDS-PAGE. Endotoxin: ≤0.1ng/ug (IEU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.5. Reconstitute with sterile ddH2O, 0.1% BSA to a concentration of 0.1-1mg/ml. Do not vortex.
Extra Details:: GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant human GM-CSF is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.48kD. Amino Acid Sequence: MAPARSPSP STQPWEHVN AIQEARRLL NLSRDTAAE MNETVEVIS EMFDLQEPT CLQTRLELY KQGLRGSLT KLKGPLTMM ASHYKQHCP PTPETSCAT QIITFESFK ENLKDFLLV IPFDCWEPV QE Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤0.1 ng/ml, corresponding to a specific activity of ≥1x10e7units/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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