G8950-05H

Granulocyte Colony Stimulating Factor (G-CSF)

Size

£403.00

SKU:: G8950-05H
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: E. coli-expressed recombinant human G-CSF
Clone:: BVD13-3A5
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: Applications: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1, 2 Purified antibody should be paired with biotinylated antibody (Cat. No. G8950-05E) as the detection antibody, with purified recombinant human G-CSF as the standard. Western Blot: Useful for Western blot applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100ng/lane of recombinant human G-CSF under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.