G8950-03A

Granulocyte Colony Stimulating Factor (G-CSF)

Size

£668.00

SKU:: G8950-03A
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse G-CSF, expressed in E.coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. No preservatives added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Granulocyte Colony Stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: ≥0.5ug/ml by using 100ul/well. Western Blot: 0.1–0.2ug/ml. Detects 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.005-0.010ug/ml yields one-half maximal inhibition (ND50) of the biological activity (50ng/ml mG-CSF). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.