Gq, 11 alpha, CT (GNA11, GA11)

Size

£837.00

SKU:: G8700-05
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa350-359, QLNLKEYNLV of G11a or Gqa, conjugated to KLH. This sequence is highly conserved among species. Species Sequence Homology: mammalian
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:2000 detects Gqa and G11a in 20ug of rat brain microsomal preparation. A previous lot detected Gqa and G11a in 20ug mouse brain microsomes and membranes and Gq/11a in 10ug of mouse 3T3/NIH fibroblast soluble plasma membranes. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G8700-05. Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Gqa and G11a. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice