G8600-79L
GPR75 (GPCR75, G-Protein Coupled Receptor 75)
Size
£825.00
- SKU:
- G8600-79L
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0 mouse myeloma cell line transfected with human GPR75.
- Clone:
- 11n81
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GPR75 is a 540aa, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75aa sequence identity with the corresponding regions of mouse and rat GPR75. Applications: Suitable for use in Direct ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml for immersion fixed paraffin-embedded sections of human brain (hippocampus) subjected to heat-induced epitope retrieval. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice