G8600-40
G-Protein Coupled Receptor 34 (GPCR34, GPR34)
Size
£832.00
- SKU:
- G8600-40
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- NS0 cells transfected with human GPR34. Uniprot/Accession Number: Q9UPC5
- Clone:
- 8H179
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- GPR34 is a widely expressed 75-90kD seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% aa sequence identity with mouse and rat GPR34. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 A172 human glioblastoma cells fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml using immersion fixed A172 human glioblastoma cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice